Lab 12  * Fossil record of modern fern & conifer families
 
I. Modern ferns
Earlier we looked at Paleozoic fern diversity and studied such groups as the Stauropteridales, Zygopteridales, Marattiales and Paleozoic Filicales. Most of the plants we looked at earlier are extinct today, except for the Marattiales, which have some living relatives like Marattia and Angiopteris, and, of course, the modern Filicalies, which include most of the ferns present in today's flora. We will also look at the Ophioglossales, a small homosporous family, and at the heterosporous water ferns traditionally placed in Marsilieles and Salviniales. In this lab we will look at fossil representatives of primarily modern fern groups. 
 
A. Ophioglossales
The Ophioglossales are a small order of homosporous ferns that include the three genera Ophioglossum, Botrychium, and Helminthostachys. This group is characterized by homosporous, eusporangiate plants that bear their sporangia in a fertile spike. While Ophioglossum ("Moonwort") has a simple, entire frond, Botrychium ("Grape fern") has a highly dissected pinnately organized frond. In contrast, the frond of Helminthostachys is palmately compound. Look briefly at the herbarium sheet of Botrychium. Although the fossil record of this group is scarce, excellent fossils of Botrychium have recently been reported from the Paleocene of Alberta, Canada. See text for details (p. 235-236). 
 
B. The Modern Filicales
The Filicales are the so-called "true ferns" and represent the major groups of ferns today. Filicaleans are homosporous and bear a relatively small number of spores in reduced sporangia called leptosporangia. Leptosporangia are stalked and have an annulus, a group of larger, thicker-walled cells on a portion of their surface. It is the position of the annulus, the arrangement of the sori (clustered sporangia) and the presence or absence of accessory structures that provide the means for classification of the filicaleans. We will look at a some Pennsylvanian filicalean ferns and then a few representatives of filicalean ferns that have extensive fossil records. Many of the modern filicalean fern families have a long fossil record. We will consider several of them. 
 
1. Osmundaceae
The Osmundaceae are a group of primitive filicalean ferns that are found in anatomically preserved state as far back as the Permian. Numerous species of Osmunda, Osmundicaulis, and several other genera have been described based on variations in anatomical detail of petiole and rhizome anatomy. Look first at herbarium sheets and photographs of the Osmundaceae. This family includes Osmunda cinnamomea (Cinnamon fern), O. claytoniana (Interrupted fern), and O. regalis (Royal fern). Now look at a transverse section of the Osmunda rhizome. This fern produces numerous leaves closely together, such that there are numerous leaf, as well as root traces visible in the axis. This stem has an ectophloic siphonostele, and contains a large amount of sclerenchyma in the pith and cortex, which stains red or purple in prepared slides. Note the protoxylem strands that are mesarch, mixed in with the rest of the primary xylem. Fossils of the Osmundaceae are found in anatomically preserved state as far back as the Permian. Numerous species of Osmunda, Osmundicaulis, and several other genera have been described based on variations in anatomical detail of petiole and rhizome anatomy. Most of the fossil material that has been described for this group is of vegetative material. Is there a problem in using this type of material to talk about evolution in this group? 
 
Look at the specimens of osmundaceous fossil stems in the lab. 
 
The genus Tempskya represents petrified trunks of "false stems" that are actually composed of a large number of individual rhizomes that grow together. Tempskya is found in Cretaceous strata in North America and is an extinct form. Look at the sections of Tempskya. Can you identify individual axes?
2. Gleicheniaceae
The Gleicheniaceae are primarily tropical ferns that have long creeping rhizomes and large fronds that can get to be up to 10 meters long, and include the genera Gleichenia and Dicranopteris. The fronds characteristically are forked with foliar buds occurring at the junctures. Stems of Gleichenia are protosteles with a mixed pith. Sori commonly have 2 -4 large sporangia and lack indusia. The annulus is transverse to oblique. Generally this family is considered to be a fairly primitive group that is related to fossil forms of Jurassic and Cretaceous, and possibly Pennsylvanian age. Look at the herbarium sheets of the gleicheniaceous Malaysian fern Dicranopteris. Compare with the fossil foliage of Gleichenia from the Cretaceous of Utah.
3. Matoniaceae
The Matoniaceae, including the extant genus Matonia and the Triassic/Jurassic fossil form Phlebopteris are today tropical plants from Indonesia, New Guinea and Borneo. The fronds are forked and have pinnatifid organization. The rhizome has concentric vascular cylinders with amphiphloic phloem. Sori are composed of relatively few, large sporangia that are protected by a conspicuous peltate (umbrella-like) indusium. The annulus is oblique, stalks are short, and sporangia in a given sorus mature simultaneously. Spores are smooth and triangular with a conspicuous trilete mark. Look at the fossil foliage of Matondium. The Matoniaceae are thought to have an evolutionary relationship to the Gleicheniaceae, on the basis of reproductive structures. 
 
4. Dennstaeditioideae/Asplenioideae
The genus Onoclea is placed within this complex which is characterized by ferns with erect to long, creeping stems with trichomes, scales or both. 
 
Compare the herbarium sheet of extant Onoclea to the photographs of fossil Onoclea found in the Paleocene of Alberta, Canada. 
 
 C. The heterosporous water ferns: Marsilieles and Salvineales
 
1. Marsileales
The Marsileales are comprised of three genera, Marsilea, Regnellidium and Pilularia. All of these plants live along pond or lake margins and produce a creeping rhizome that bears the fronds and roots at nodes. Marsilea has a 4-lobed pinnule and resembles a 4-leaf clover, while in Regnellidium the frond is 2-lobed and Pilularia lacks a laminate portion to its frond and has a reduced, grass-like leaf. Rhizomes are amphiphloic siphonosteles, with both inner and outer phloem, frequently zonate cortex, with an inner, more compact zone and an outer aerenchymatous zone, and may have a sclerotic pith. The reproductive structures of the Marsileales are called sporocarps: these are small bean-like structures borne singly or on branches at the base of fronds, and are produced during adverse conditions, as water availability becomes scarce. They are interpreted as being homologous to either a single pinna or an entire frond. When they are moistened, sporocarps open to produce an elongate, gelatinous structure called a sorophore that bears a row of sori, each in an indusium. The fossil record of the Marsileales is not very extensive. However, in a recent publication by Skog and Dilcher, plants of this group have been described from the Cretaceous of Kansas. 
 
2. Salvineales 
In contrast to the Marsileales, the Salvineales are completely floating plants. There are two genera, Azolla and Salvinea. Salvinea has a rhizome that bears leaves in whorls of three, with two of each whorl being thick, flattened photosynthetic leaves that have a thick pad of hairs on their abaxial surface that maintain them as floating plants, and a third leaf that is highly dissected, submerged and acts as a root. There are no true roots in this plant. In Azolla, the leaves are spoon-shaped and fit into one another, and true roots are present. In these two genera the reproductive structures are sporocarps that are basically modified indusia. Within each sorus, either the megasporangium with its one functional megaspore, or a cluster of microsporangia with numerous microspores differentiates, but not both. Along with the spores, tissue derived from the tapetum forms an accessory tissue that produces massulae or "floats". As the spores emerge from their sporangia the attached floats act to maintain them as floating units. Floats containing embedded masses of microspores often have tiny hook-like projections called glochidia that hook onto the megaspore allowing for fertilization of the archegonia within the megagametophyte tissue within the megaspore. Azolla also harbors the blue-green nitrogen fixing alga Anabaena in a symbiotic relationship, and thus has been economically important, especially to rice production.
The distinctive megaspores, as well as whole plants of Azolla, have been found rather extensively in a number of Cretaceous and Tertiary fossil deposits. Look at the herbarium sheets of Azolla and compare to fossils of from British Columbia.
Since the text was published, an interesting aquatic fern has been described that has features of both Marsileales and Salvineales and suggests that these two orders are more closely related than was previously thought. See the paper on Hydropteris by Rothwell and Stockey. 
 
II. The Origin of Modern Conifer Families 
The families of modern conifers are delimited primarily on the following characters: leaf arrangement and morphology, degree of fusion of ovulate bract-scale complex, number of ovules per scale, number of pollen sacs per microsporophyll, and type of pollen. Below is a brief summary of each family and its fossil record. 
1. Araucariaceae:
This southern hemisphere family is represented today by the genera Araucaria (Norfolk Island pine) and Agathis (monkey puzzle tree). These plants are either dioecious or monoecious trees with opposite or spirally arranged leaves that are linear to broad. Pollen cones have 5-20 pendant microsporangia per microsporophyll while seed cones contain one ovule per fused bract-scale complex. Pollen is nonsaccate.
Araucarian conifers have a rich fossil record, extending from at least the Triassic, on the basis of reproductive structures. The genus Araucarites, in particular, represents fossil specimens of fused bract-scale complexes similar to those of extant Araucaria. (See the diagram on p. 425). As is discussed in the textbook, wood and leaf fossils with features similar to modern Araucariaceae may point to earlier origin but cannot be unequivocally placed in this group. Permineralized Jurassic cones are well known and contain young embryos. 
Although the araucarians are restricted today to the southern hemisphere, fossil evidence from the Mesozoic demonstrates that they were widespread throughout the world from the Triassic through the Cretaceous. Their present day distribution probably dates back to the Tertiary.
Look briefly at the Jurassic Araucaria cone and at the paper by Stockey on the history of the araucarians. 
 
2. Podocarpaceae: 
Podocarpus, Dacrydium, Phyllocladus are genera in this second, primarily southern hemisphere family. These plants are mostly dioecious trees or shrubs with spirally arranged, scalelike, linear or broad leaves. Pollen cones have 2 microsporangia per microsporangium while seed cones have one ovule maturing per ovulate structure that is conelike or modified into several small scales and a subtending bract. The ovule may be borne on fleshy "receptacle" (epimatium) at maturity. Pollen is saccate. 
 
In contrast to the araucarians, which had a wide distribution in the Mesozoic, the podocarps have always been essentially southern hemisphere plants. Podocarps are first recognized in the Triassic, and become extensive in the Jurassic of Gondwana. Vegetative and reproductive structures referred to the genus Rissikia from Australia and Africa are podocarpaceous, as are several Jurassic forms. Recently a podocarpaceous stem has been described from the Triassic of Antarctica.
Look at the photographs of Rissikia in the book by White (p. 160-2). 
 
3. Pinaceae:
The Pinaceae are the best known and largest family of extant conifers, and are probably the most familiar to you because they are a predominantly northern hemisphere group well represented in the current flora. This family is represented by the genera Pinus (pine), Pseudotsuga (Douglas fir), Abies (fir), Picea (spruce), Cedrus (cedar). These plants are monecious trees with leaves that are spirally arranged, linear or needlelike. Pollen cones in this group have 2 microsporangia per microsporophyll while seed cones bear 2 ovules per woody cone scale, and have scales and bracts that are free from one another. Pollen is bisaccate.
The fossil record of the Pinaceae extends from at least the Cretaceous based on anatomically preserved cones. However, a number of Triassic and Jurassic compressed fossils that are less completely known have also been suggested as members of the Pinaceae. In the Cretaceous, numerous pinaceous cones are found anatomically preserved. Although a few can be assigned to the modern genus Pinus, most are placed in the fossil genus Pityostrobus because they combine characters not found in modern taxa. Other modern pinaceous genera evolved during the Tertiary.
Look at specimens of Pinus ovulate cones, stems and leaves from the petrified Princeton Chert, and at the compressed Miocene pine needles from Creede, Colorado.
4. Taxodiaceae
The Taxodiaceae are another good example of a family of plants that was widely distributed and common in the Cretaceous and Tertiary, that has a much more restricted distribution today. This family is represented by Taxodium (bald cypress), Metasequoia (dawn redwood); Sequoia (redwood), Sequoiadendron (giant redwood), and Cryptomeria. These plants are monecious trees with leaves that are spirally arranged or opposite, needlelike or linear. Pollen cones have 2-9 microsporangia per microsporophyll while seed cones bear 2-9 ovules per scale and have scales and bracts that are joined. Pollen is nonsaccate.
The fossil record of the Taxodiaceae extends from the Jurassic to the Recent. Fossil representatives are plentiful especially in the Cretaceous when Metasequoia was widespread and represents the dominant foliage type in many localities, particularly in northwestern North America. 
Look at compressed Metasequoia foliage from the Cretaceous of Alaska and at the paper by Serbet and Stockey on cones from Drumheller, Alberta. 
 
A fossil family, the Cheirolepidaceae, which may be transitional between the Voltziales and Taxodiaceae is important from the Triassic through the Cretaceous in Europe and Argentina. This family is a good example of the greater degree of conifer diversity that occurred during the Mesozoic. We know this group was widespread and ecologically important because of both the megafossil record and its distinct pollen, Classopollis, which rivals the angiosperms in complexity of pollen wall structure.
5. Cupressaceae:
Cupressus, Juniperus (juniper), Thuja (hemlock) are included in this family. These plants are monoecious or dioecious trees and shrubs with leaves that are either opposite or whorled and usually scalelike. Pollen cones have 3-6 (+) microsporangia per microsporophyll while seed cones have 2-many ovules borne on fused bract-scale complex. Pollen is nonsaccate.
The fossil record of cupressaceous plants is less detailed than for a number of the families discussed above. One problem is that it is difficult to determine whether some foliage is cupressaceous or taxodiaceous based on whether leaves are borne decussately or helically. This family is at least as old as the Jurassic, with extant genera recognizable by the Upper Cretaceous.
6. Cephalotaxaceae:
This family is represented by the genus Cephalotaxus. These plants are mostly dioecious trees or shrubs with spirally arranged to two-ranked, needlelike leaves. Pollen cones contain 3-8 pollen sacs per microsporophyll and seed cones are decussately arranged with 2 ovules per bract. Only one ovule matures into an olive-like seed. Pollen grains are nonsaccate.
The fossil record of this group is relatively small, but extends from the Jurassic to the Recent. While in extant Cephalotaxaceae single ovules develop from cones, fossil relatives such as Palissya show numerous ovules on the bract-scale complex. See text for details.
7. Taxales 
 
A. Taxaceae: Taxus (yew), Torreya; Trees or shrubs dioecious; leaves spirally arranged, linear or needlelike; 2-8 microsporangia per peltate microsporophyll, one ovule at maturity on ovuliferous branch enclosed in a fleshy aril; pollen nonsaccate.
Taxaceous plants are first reported from the Jurassic. Their relationships to the transition conifers are less clear than are those of other families.